KMID : 0613820200300040343
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Journal of Life Science 2020 Volume.30 No. 4 p.343 ~ p.351
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Inhibitory Effect of Scopoletin Isolated from Sorbus commixta on TNF-¥á-Induced Inflammation in Human Vascular Endothelial EA.hy926 Cells through NF-¥êB Signaling Pathway Suppression
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Kang Hye-Ryung
Kim Hyo-Jung Kim Bo-Mi Kim Sun-Gun So Jai-Hyun Cho Soo-Jeong Kwon Hyun-Sook
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Abstract
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Sorbus commixta Hedl. has traditionally been used as a remedy for cough, asthma, and other bronchial disorders. In this study, three major triterpenoids?lupeol, ¥â-sitosterol, and ursolic acid and a coumarin, scopoletin, were isolated from a CHCl3-soluble fragment of the bark of S. commixta. Their structures were identified by spectroscopic analyses, including mass spectrometry (MS), 1D-, and 2D- nuclear magnetic resonance spectroscopy (NMR), as well as by comparing the data with data reported in the literature. Scopoletin was isolated from this plant for the first time. It is a nutraceutical compound contained in many plants that has been reported to exert diverse biological activities, including anti-inflammatory effects. This study examined the inhibitory effect of scopoletin on TNF-¥á-induced vascular endothelial inflammation. Unlike the marginal impact of other compounds against low-density lipoprotein (LDL) oxidation and vascular endothelial inflammation, scopoletin showed remarkable activity on LDL oxidation (IC50 = 10.2 ¥ìM) and exerted vascular anti-inflammatory effects in EA.hy926 human endothelial cells activated by TNF-¥á. It suppressed the expression of adhesion molecules, such as ICAM-1, VCAM-1, and E-selectin, and blocked the adhesion between THP-1 monocytes and EA. hy926 endothelial cells. It also inhibited TNF-¥á-induced NF-¥êB translocation from the cytosol to the nucleus. Moreover, I¥êB¥á phosphorylation, which was increased by TNF-¥á treatment, was reduced after treatment with scopoletin. Thus, scopoletin inhibited TNF-¥á-induced vascular inflammation in endothelial cells by suppressing the NF-¥êB signaling pathway. These results demonstrate that owing to its anti-inflammatory activity in the vascular endothelium, scopoletin has the potential to inhibit atherosclerosis development.
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KEYWORD
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Adhesion molecules, inflammation, scopoletin, Sorbus commixta
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